International Journal of Animal Biotechnology and Applications
Relative Accuracy of Diagnostic Methods in Verification of Gonadal Status of Dogs Considering Anti-Mullarian Hormone as a Biomarker
1. Department of Veterinary Gynaecology and Obstetrics, Veterinary College (KVAFSU), Hebbal, Bengaluru, Karnataka, India
2. Professor,Department of Veterinary Public Health and Epidemiology,Veterinary College (KVAFSU), Hebbal, Bengaluru, Karnataka, India.
3. Assistant Professor,Department of Veterinary Physiology,Veterinary College (KVAFSU), Hebbal, Bengaluru, Karnataka, India.
4. Department of Animal Genetics and Breeding,Veterinary College (KVAFSU), Hebbal, Bengaluru,
Karnataka, India,
5. Department of Veterinary Pathology, Veterinary College (KVAFSU), Hebbal, Bengaluru, Karnataka, India
Abstract
The study was carried out to demonstrate relative efficacy of various methods to ascertain gonadal status in dogs. Based on different diagnostic methods viz., history and clinical signs, physical examination and ultrasonography, 37 (21 male and 16 female) dogs were categorised into different groups. Group I- gonadectomised dogs (n=10; Male-5 Female-5), Group II - intact dogs before and after gonadectomy (n=16; Male-8 Female-8), Group III-dogs with ovarian remnant syndrome (ORS) (n=3) and Group IV- dogs with either unilateral/ bilateral cryptorchidism (n=8). All the dogs were subjected to serum Anti- mullarian hormone (AMH) estimation using canine specific AMH kits. The mean AMH level in intact male dogs, castrated and cryptorchid dogs was 16.20 ± 1.44, 0.57 ± 0.06 and 24.90 ± 0.79 ng/mL, respectively. In female dogs, the mean AMH value was 5.14 ± 0.54, 3.20 ± 1.54 and 0.26 ± 0.02 ng/mL in intact, ORS and spayed dogs, respectively. By considering AMH as the standard to ascertain the gonadal status, the relative accuracy of history and clinical signs was 85.71 and 100%, physical examination was 61.90 % and 43.75 %, whereas with trans- abdominal ultrasonography 71.42% and 18.75% in male and female dogs, respectively. A single serum AMH concentration measurement was highly effective in accurately distinguishing between gonadectomized and intact adult dogs.. In addition, this test also accurately identified several cases of ovarian remnant syndrome and cryptorchidism which were failed to identify using other methods.
Key words: Dogs, gonadectomy status, AMH, cryptorchid, ORS, ultrasonography
References (1)
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