Optimization of Nutritional Parameters for Production of Alpha Amylase Using Aspergillus oryzae MTCC 3017 by Central Composite Design

P Mary Anupama; Subba Rao Somalanka

International Journal of Industrial Biotechnology and Biomaterials

Optimization of Nutritional Parameters for Production of Alpha Amylase Using Aspergillus oryzae MTCC 3017 by Central Composite Design

Abstract

Amylases are the most widely used enzymes that are used many sectors such as clinical, medical, analytical and industrial applications. Beside their use in starch saccharification they also find applications in food, baking, brewing, detergent, textile and paper industries. Increasing utility and consumption of amylase in different industries has placed a greater stress on increasing indigenous enzyme production and search of more rapid processes. The aim of the present investigation is to achieve optimal amylase production by using the culture Aspergillus oryzae MTCC 3107. Central composite design was used to optimize the condition for the six independent variables Starch, Peptone and the four mineral elements. The critical values obtained at the end of experimentation resulted in amylase yield of 4496.72 units while the expected was 4116.37 units. The critical condition revealed, when investigated had resulted in a 9.24% increase in product concentration. An R2 value of 0.96172 indicates 96% fit of the model. This states that the chosen statistical model for the production of amylase is a powerful to get good product yield.

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How to cite

P Mary Anupama, Subba Rao Somalanka. Optimization of Nutritional Parameters for Production of Alpha Amylase Using Aspergillus oryzae MTCC 3017 by Central Composite Design. International Journal of Industrial Biotechnology and Biomaterials. 2016;2(1).

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Optimization of Nutritional Parameters for Production of Alpha Amylase Using Aspergillus oryzae MTCC 3017 by Central Composite Design

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