Development and Validation of a Reversed-Phase HPLC Method for the Simultaneous Determination of Telmisartan and Its Related Compound B (Positional Isomers) in the Bulk Drug

Kamlesh M. Patel; Upendra R. Patel; Ashaben D. Patel

International Journal of Analytical and Applied Chemistry

Development and Validation of a Reversed-Phase HPLC Method for the Simultaneous Determination of Telmisartan and Its Related Compound B (Positional Isomers) in the Bulk Drug

Abstract

A simple, selective, accurate, precise and linear RP-HPLC method was developed and validation for Simultaneous determination of Telmisartan and its related Compound B 4ʹ-[(1,7-Dimethyl-2'-propyl[2,5'-bi-1H-benzimidazol]-1'-yl)methyl][1,1'-biphenyl]-2-carboxylic acid; 4'-[(1,7'-dimethyl-2'-propyl-1H,1'H-2,5'-bibenzo[d]imidazol-1'-yl)methyl]biphenyl-2-carboxylic acid; was accomplished in the bulk drug and synthesis for drug and a tablet formulation using a Reversed Phase High Performance liquid chromatography method with UV detection separation was achieved on Inertsil ODS-3V column (150 × 4.6 mm, 5 μm) (GL Science) as stationary phase with binary gradient mode solvent phase A. composed of KH2PO4 (potassium dihydrogen orthophopsphate) buffer (PH adjust =3.5, with triethylamine) and phase B of use filter and degas acetonitrile. The flow rate of the mobile phase was 1.0 mL/min and the total elution time including the column re-equilibration was approximately 30 min. The UV detection wavelength was 246 nm, injection volume was 10 μL and experiments were conducted at 30 °C temperature. The developed method was validated in terms of system suitability, selectivity, linearity, range, precision, accuracy, limits of detection and quantification the for the related compound B, short term and long term stability of the analysts in the prepared solution and robustness, following the ICH guidelines therefore the proposed method was suitable for the simultaneous determination of telmisartan and its related compound B. Keywords: binary gradient, degas acetonitrile, precision, system suitability, telmisartan

Full text

The full text of this article is available to subscribers.

On a subscribing campus you are let in by network address, with no login at all.

One-off purchase. Permanent access to this article, no account needed.

A year of online access to every article in International Journal of Analytical and Applied Chemistry. Or choose several journals →

How to cite

Kamlesh M. Patel, Upendra R. Patel, Ashaben D. Patel. Development and Validation of a Reversed-Phase HPLC Method for the Simultaneous Determination of Telmisartan and Its Related Compound B (Positional Isomers) in the Bulk Drug. International Journal of Analytical and Applied Chemistry. 2020;1(2):12–23.

Download the citation:

Article details

Development and Validation of a Reversed-Phase HPLC Method for the Simultaneous Determination of Telmisartan and Its Related Compound B (Positional Isomers) in the Bulk Drug

Abstract

Read more of it

Publish your article

Abstract

Related articles

Cryogenic Electron Microscopy Reveals Conformational Dynamics and Catalytic Mechanisms of Human Fatty Acid Synthase in De Novo Lipid Biosynthesis

General and Organic Chemistry Laboratory Experiments for Science, Pre-medical, and Pharmacy Students – Chapter V: Organic Synthesis

Photo-Catalytic Degradation of Dyes Using Graphene Oxide–Dithizone Composite

Spatial Variation and Pollution Assessment of Selected Water Quality Variables in the Luubara Creek, Niger Delta, Nigeria

Chemistry of Mehandi (Henna): Natural Dyeing Mechanism, Composition, and Applications

Agro Seed Oil Production through Mechanothermal Extraction: Exploring its Potential for Chemical, Pharmaceutical, and Food Industries

Read more of it

Publish your article