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13 articles for “in vitro culture”
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In Vitro Culture and Water Stress Tolerance Assessment in Banana (Musa Paradisiaca L.)
Abstract: This study investigates the in vitro culture and water stress tolerance of Musa paradisiaca L. (Banana) using a systematic approach to explant preparation, sterilization, media preparation, and stress induction. Banana explants were sourced locally and treated to ensure aseptic conditions. Murashige and Skoog (MS) medium, enriched with essential nutrients, vitamins, and plant growth hormones (IAA and BAP), served as the base for culturing. The study applied Polyethylene Glycol (PEG-6000) as …
Published in International Journal of Plant Biotechnology · Vol. 11, Issue 1, 2025 · pp. 42–53 Read article
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Plant Tissue Culture: Conservation Techniques
Abstract: Agriculture, forestry, horticulture, and plant breeding have all made extensive use of plant tissue culture. It's a type of applied biotechnology that's utilized for things like plant mass propagation, viral eradication, secondary metabolite manufacturing, and in-vitro cloning. Tissue culture has increasingly been employed for short to medium term conserving, also known as slow development, and cryogenics, also known as long term conservation, of vulnerable species of plants. These techniques had …
Published in International Journal of Plant Biotechnology · Vol. 8, Issue 1, 2022 · pp. 27–29 Read article
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In-vitro plant regeneration of Mulberry (Morus alba L.)
Abstract: The present investigation entitled “In-vitro plant regeneration of Mulberry (Morus alba L.)” was conducted at Plant Tissue Culture Laboratory of K. K. Wagh College of Agricultural Biotechnology, Nashik, Maharashtra, India. Development of improved protocols for the regeneration of Morus alba L. is important for their commercial utilization. The present study was initiated to optimize in-vitro propagation protocol from nodal culture of Mulberry. Three surface sterilization treatment were used, the nodal …
Published in International Journal of Plant Biotechnology · Vol. 9, Issue 1, 2023 · pp. 24–38 Read article
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Animal Cell Culture: Features, Requirements and Various Applications
Abstract: Animal cell culture fundamentally includes the in vitro (in the laboratory) support and proliferation of animal cells in a suitable nutrient media. In this manner, culture is a procedure of developing cells falsely. Cell culture has turned into a key innovation in different branches of life sciences.
Published in International Journal of Animal Biotechnology and Applications · Vol. 2, Issue 2, 2016 · pp. 19–24 Read article
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Cell Culture Technologies in Animal Biotechnology: Innovations and Applications
Abstract: Cell culture technologies have become integral to animal biotechnology, enabling the in vitro study of cellular behaviors, disease mechanisms, and the development of therapeutic solutions. These technologies are foundational for advancements in drug discovery, gene therapy, regenerative medicine, and vaccine production. Over recent decades, innovations in culture media, bioreactor systems, and three-dimensional (3D) cell culture models have significantly enhanced the efficiency and reproducibility of cell-based research. Serum-free and chemically defined …
Published in International Journal of Animal Biotechnology and Applications · Vol. 10, Issue 2, 2024 · pp. 25–29 Read article
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Synthetic Seed Technology and Its Applications: A Review
Abstract: Synthetic seed technology is a novel approach in plant tissue culture, as it joins the benefits of clonal propagation with those of seed proliferation. Production of synthetic seeds has opened new era in in vitro plant propagation technology, because it offers many useful advantages on a commercial scale for the propagation of a variety of plants. Synthetic seed technology is useful in multiplication and conservation of elite plant species, which …
Published in International Journal of Plant Biotechnology · Vol. 3, Issue 1, 2017 · pp. 11–16 Read article
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Antagonistic Activity of Fungi and Bacterium against Red Rot Disease of Saccharum officinarum: An Approach for Biocontrol
Abstract: The Two species of fungus Trichoderma harzianum and Aspergillus flavus and one species of bacterium viz. Bacillus subtilis were tested for antagonistic activity in vitro against Colletotrichum falcatum, pathogen of red rot disease of sugarcane. Two Saccharum officinarum cultivars viz. CoS 98231 and CoLK 8102 in pot and field experiments, by a dual culture technique. It was found that isolates of fungal and bacterial inhibited the mycelial growth of C. …
Published in International Journal of Plant Biotechnology · Vol. 2, Issue 2, 2016 · pp. 14–18 Read article
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In Vitro Callus Induction of White Pumpkin (Cucurbita moschata Duch.) ‘Casperita’ (F1 Hybrid) through Difference Explants
Abstract: White pumpkin (Cucurbita moschata Duch.) ‘Casperita’ (F1 Hybrid) is a popular pumpkin in many countries, however, it produces unfertile seeds. The current study determined the appropriated concentrations of 2,4-D (2,4-dichlorophenoxy acetic acid) and BAP (6-Benzylaminopurine) on callus induction using various explants as hypocotyl, epicotyl, cotyledons, petiole, and leaf. Sterilized explants were cultured on MS media with various concentrations of 2,4-D (0 and 2 mg/L) and BAP (0, 0.1, 0.3 and …
Published in International Journal of Plant Biotechnology · Vol. 5, Issue 2, 2019 · pp. 15–21 Read article
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Extraction, Characterization and Tissue Culture of Plant Derived Indigo From Indigofera tinctoria-Preliminary Studies
Abstract: Indigo is the most preferable of all natural dyes for its high demand worldwide because of the blue denim. Natural Indigo dye is eco friendly, non carcinogenic and do not pose hazard to the environment as compared to synthetic dyes. Although a growing demand for naturally derived indigo dye exists, with increased use of synthetic dyes the plant species is now on the verge of being endangered. Plant tissue culture …
Published in International Journal of Agrochemistry · Vol. 4, Issue 2, 2018 · pp. 53–58 Read article
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Role of Bioactive Compounds on Protein Utilization in Ruminants: A Review
Abstract: Insufficient nutrition is the primary drive of low productivity by ruminants. These insufficiencies can be corrected by supplementation with high-density feeds and locally available tree legumes. These tree legumes contain various types of bioactive compounds, which can be defined as secondary plant metabolites (PSM) that are extra nutritional substances having pharmacological or toxicological effects in man and animals. Besides the primary biosynthetic, the secondary metabolites are also produced within the …
Published in International Journal of Animal Biotechnology and Applications · Vol. 4, Issue 1, 2018 · pp. 21–26 Read article
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Synthesis and Evaluation of Antifungal Properties of 2-((4-((6phenylimidazo[2,1-b][1,3,4]thiadiazole-2-yl)methoxy)phenoxy)methyl)-6-phenylimidazo[2,1-b][1,3,4] thiadiazole Derived from Hydroquinone
Abstract: Thiadiazole, a heterocyclic core, has garnered significant interest from chemists seeking novel therapeutic agents. The synthetic route for the target compounds is depicted in Scheme 1. The compounds were synthesized with favorable yields through the condensation of hydroquinone with various organic reagents. They underwent physical characterization and were analyzed using spectroscopic methods, including infrared (FTIR) and NMR (1H, 13C) spectroscopy. The FT-IR spectrum of the product exhibited a ν(C=N) absorption …
Published in International Journal of Environmental Chemistry · Vol. 12, Issue 1, 2026 · pp. 76–82 Read article
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In Vitro Evaluation of Bioremediation Capacity of a Commercial Probiotic, Bacillus clausii, for Chromium (VI) and Lead (II) Toxicity
Abstract: Heavy metals cause disastrous effects on any living organisms. Probiotics have the ability to reduce metal toxicity. Commercial probiotics having bioremediation capacity can use these bacteria as an alternative to conventional remediation methods. The bioaccumulation of heavy metals including chromium (VI) (Cr [VI]) and lead (II) (Pb [II]) causes fatal toxicity in humans. Some naturally occurring bacterial species, such as Bacillus and Pseudomonas, contribute their role in bioremediation of these …
Published in International Journal of Industrial Biotechnology and Biomaterials · Vol. 4, Issue 2, 2018 · pp. 1–16 Read article
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Mass Production and Isolation of Trichoderma harzianum from Different Media Using Liquid and Solid Fermentation Technique and Evolution against Fusarium oxysporum
Abstract: Mycelial growth conidal production and biomass yield of trichoderma species (Trichoderma harzianum) were examined on different culture media including potato dextrose media, waksman media, soyabean meal media, glucose peptone media, jaggery media, malt extract media, cornmeal media, czepaks media. A big factor in growth rate was the medium. The optimum medium in terms of growth spore generation and biomass output was soyabean meal media. For mycelial growth, biomass output, and …
Published in International Journal of Chemical Separation Technology · Vol. 8, Issue 1, 2022 · pp. 27–37 Read article