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6 articles for “Cryopreservation”
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Cryopreservation of Embryo
Abstract: Cryopreservation or cry conservation is a procedure where organelles, cells, tissues, extracellular matrix, organs or whatever other natural develops powerless to harm brought on by unregulated chemical kinetics are protected by cooling to low temperatures[1](typically - 80 °C utilizing strong carbon dioxide or - 196 °C utilizing fluid nitrogen). At sufficiently low temperatures, any enzymatic or chemical activities which may make harm the biological material being referred to be viably …
Published in International Journal of Animal Biotechnology and Applications · Vol. 2, Issue 2, 2016 · pp. 12–14 Read article
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Cryopreservation
Abstract: Cryopreservation depends on the capacity of certain molecules to enter cells and avert drying out and arrangement of intracellular crystals, which can bring about cell death and annihilation of cell organelles amid the solidifying procedure. Two basic cryoprotective operators are dimethyl sulfoxide (DMSO) and glycerol. Glycerol is utilized fundamentally for cryoprotection of red platelets, and DMSO is utilized for assurance of most different cells and tissues. A sugar called trehalose, …
Published in International Journal of Animal Biotechnology and Applications · Vol. 2, Issue 1, 2016 Read article
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Plant Tissue Culture: Conservation Techniques
Abstract: Agriculture, forestry, horticulture, and plant breeding have all made extensive use of plant tissue culture. It's a type of applied biotechnology that's utilized for things like plant mass propagation, viral eradication, secondary metabolite manufacturing, and in-vitro cloning. Tissue culture has increasingly been employed for short to medium term conserving, also known as slow development, and cryogenics, also known as long term conservation, of vulnerable species of plants. These techniques had …
Published in International Journal of Plant Biotechnology · Vol. 8, Issue 1, 2022 · pp. 27–29 Read article
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Advances in Reproductive Biotechnology: Innovations in Artificial Insemination and IVF Techniques
Abstract: Reproductive biotechnology has transformed infertility management and animal breeding, with artificial insemination (AI) and in vitro fertilization (IVF) playing pivotal roles. Historically, these technologies have evolved from experimental concepts to essential tools in reproductive medicine and agriculture. AI, introduced in the early 20th century, became instrumental in optimizing breeding programs by allowing selective fertilization without physical mating, thus improving genetic diversity and disease control in livestock. Meanwhile, IVF, developed in …
Published in International Journal of Animal Biotechnology and Applications · Vol. 10, Issue 2, 2024 · pp. 11–15 Read article
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Common Methods for Isolation, Revival and Freezing of Peripheral Animal Blood Mononuclear Cells—A Review
Abstract: ABSTRACTThe main players to investigate to effect of bio actives on various immune cells are human peripheral blood mononuclear cells (PBMCs). These cells are easily isolated from blood of healthy donors or buffy coats (leukocyte concentrates, a by-product from hospital Blood Banks in the manufacturing of red blood cell and thrombocyte concentrates from anti-coagulated whole blood). PBMCs vary from intestinal tissue cells in terms of structure, phenotype, and activation status. …
Published in International Journal of Animal Biotechnology and Applications · Vol. 6, Issue 2, 2020 · pp. 21–23 Read article
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Stem Cells and their Applications
Abstract: Stem cells have a unique ability to develop into specialized cell types in a body that can be used to replace cells and tissues. They are classified according to their sources and potency. Stem cell therapies play an important role in cell transplantation biology. There are 3 basic types of transplants. These are called autologous, allogeneic, and syngeneic. Stem cells treatment is used in treating burned wounds, skin replacement, brain …
Published in International Journal of Immunological Nursing · Vol. 6, Issue 2, 2020 · pp. 6–14 Read article