Pitaya Cactus Endophytic Bacteria a Sustainable Option to Reduce and Optimize NH4NO3 in Phaseolus vulgaris

Juan Manuel Sánchez Yáñez; Juan Luis Ignacio-De la Cruz; Guidier Marto-Domínguez; Ramiro Eleazar Ruiz-Nájera

Abstract

The Phaseolus vulgaris crop demands nitrogen fertilizer as NH4NO3 for healthy growth. However, the application of NH4NO3 not uptaken by plant roots, generates N2O, a gas that contributes to the greenhouse effect besides loss of soil fertility; a sustainable option is to reduce up to 50% and to optimize it by inoculating P. vulgaris seeds with genera and species of plant growth promoting endophytic bacteria isolated from a desert xerophyte such as Stenocereus queretaroensis tolerant to water stress and NH4NO3 dependent nutritional stress. The aims of this research were: a) to isolate Burholderia vietnamiensis and Gluconoacetobacter diazotrophicus endophytic in root of S. queretaroensis; and b) the analysis of growth of P. vulgaris with B. vietnamiensis and G. diazotrophicus. P. vulgaris seeds were infected with B. vietnamiensis and G. diazotrophicus at 50% NH4NO3 in a greenhouse trial. After that, response variables in were determined P. vulgaris: germination, phenology and biomass to seedling and flowering; these numerical data were analyzed by ANOVA and Tukey (P<0.05%). The results showed a positive response of P. vulgaris to B.vietnamiensis and G. diazotrophicus at 50% NH4NO3 in seed germination; on the phenology/biomassat seedling and flowering stages, in P. vulgaris detected 1.97 g of airborne dry weight (ADW) and 1.88 g of root dry weight (RDW), statistically different compared to 1.14 g ADW and 0.69 g RDW in P. vulgaris uninoculated with B. vietnamiensis and G. diazotrophicus, fed at 100% NH4NO3 or (relative control). These findings revealed that B. vietnamiensis and G. diazotrophicus endophytes of S. queretaroensis colonized the interior of the root system of P. vulgaris; there they converted root metabolites into phytohormones that optimized uptake of NH4NO3 at 50% without leaving any concentration of nitrogen fertilizer free, which avoids the emission of greenhouse gases andconsequently global warming.

Pitaya Cactus Endophytic Bacteria a Sustainable Option to Reduce and Optimize NH4NO3 in Phaseolus vulgaris

Abstract

Read more of it

Publish your article

Abstract

Related articles

General and Organic Chemistry Laboratory Experiments for Science , Pre-medical, and Pharmacy Students Chapter I: Introduction to Safety

General and Organic Chemistry Laboratory Experiments for Science, Pre-medical, and Pharmacy students Chapter II: Glassware, Tools and Equipments

Transportable Water and Air Quality Monitoring Equipment Karishma

Synthesis and Evaluation of Antifungal Properties of 2-((4-((6phenylimidazo[2,1-b][1,3,4]thiadiazole-2-yl)methoxy)phenoxy)methyl)-6-phenylimidazo[2,1-b][1,3,4] thiadiazole Derived from Hydroquinone

Abundance and Diversity of Meiofauna as Bioindicator of Water Quality and Health Status of Tombia Segment, New Calabar River, Niger Delta, Nigeria

Emulsion Paint Formulation from Black Seed Powder/Soya Bean Oil Copolymer Binder

Read more of it

Publish your article