Next generation bioluminescence imaging technology for tracking numerous biological processes under in vivo conditions

Sakshi Tewari; Shalini Sharma

International Journal of Animal Biotechnology and Applications

Next generation bioluminescence imaging technology for tracking numerous biological processes under in vivo conditions

Abstract

To expand our understanding on numerous biological processes happening inside living entities, the mechanism of bioluminescence exists. The process of visible light emission in living things is referred to as bioluminescence. Small laboratory animals can now be molecularly imaged using bioluminescence imaging. It is a potent technology that has been developed over the past ten years in diverse fields of science. In the area of virology, immunology, oncology, and neuroscience, this technology offers efficient ways for tracking numerous in vivo biological processes. The technique utilizes the enzyme luciferase, which is transferred inside of tissues or an entire organism, allowing for the tracking of cells, observation of gene expression, and protein-protein interactions. Despite the fact that fluorescence reporters and sensors are being used more frequently in a variety of industries, their application has been constrained by thick tissues and living entities. This problem has been solved by BLI via developing bioluminescence reporters and sensors that use enzyme-catalyzed processes to produce light emitters. The article begins with the description of diverse luciferase and luciferin pairs, followed by bioluminescent systems, their methodology, applications and ongoing trends. The paper provides details on the bioluminescence imaging methodology and its numerous applications. It emphasizes on how this method can be blended with other techniques and can serve as next generation technology for tracking numerous biological processes under in vivo conditions.

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How to cite

Sakshi Tewari, Shalini Sharma. Next generation bioluminescence imaging technology for tracking numerous biological processes under in vivo conditions. International Journal of Animal Biotechnology and Applications. 2023;8(2):21–35.

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Next generation bioluminescence imaging technology for tracking numerous biological processes under in vivo conditions

Abstract

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