Gene Cloning

Prateek Sharma

International Journal of Animal Biotechnology and Applications

Gene Cloning

Prateek Sharma ✉

Abstract

Gene cloning (DNA cloning) is a technique used for the generation of precise (clones) of a specific quality or DNA sequence utilizing genetic engineering techniques. The DNA containing the target gene(s) is cut into sections utilizing restriction enzymes. These sections are then embedded into cloning vectors, for example, bacterial plasmids or bacteriophages, which exchange the recombinant DNA to reasonable host cells, for example, the bacterium E. coli. On the other hand, complementary DNA is embedded into the vectors, or "naked" DNA pieces can be taken up directly by a host bacterium from its medium (this is less effective than vector exchange).

Full text

The full text of this article is available to subscribers.

On a subscribing campus you are let in by network address, with no login at all.

One-off purchase. Permanent access to this article, no account needed.

A year of online access to every article in International Journal of Animal Biotechnology and Applications. Or choose several journals →

Gene Cloning

Abstract

Read more of it

Publish your article

Abstract

Related articles

Animal Biotechnology: Finding a Balance Between New Ideas and Morality

Spermmediated gene transfer revisited: optimizing delivery, integration, and germline transmissionin large animal models

Stem Cell and Cloning Technologies in Livestock Improvement: Advances, Applications, and Future Prospects for Sustainable Animal Production and Genetic Improvement

Barriers to Advanced Reproductive Technologies in the Subsistence Dairy Farming Systems: Key Constraints andthe Way Forward

Barriers to Advanced Reproductive Technologies in the Subsistence Dairy Farming Systems: Key Constraints and the Way Forward

Read more of it

Publish your article