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Somatic Embryogenesis from Immature Zygotic Embryos of Sterculia alata Roxb. and Monitoring the Ontogeny and the Genetic Fidelity of Somatic EmbryosBy Madhu Tripathi, Nishi Kumari, Vijay S. Jaiswal, Uma Jaiswal, Major Singh
Abstract: A protocol for the induction of somatic embryogenesis from immature zygotic embryos of Sterculia alata was established. The highest induction frequency for somatic embryogenesis was achieved using 3 mm size of zygotic embryos as explants on Murashige and Skoog (MS) medium supplemented with 2,4-D (2.0 mgl–1) + BAP (0.1 mgl–1) and 3% sucrose. The embryogenic calli differentiated when transferred to MS basal medium (maintenance medium) after three weeks treatment in …
Published in International Journal of Plant Biotechnology · Vol. 3, Issue 2, 2017 · pp. 53–67 Read article →